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[Preprint]. 2023 Aug 4:2023.02.08.527750. Originally published 2023 Feb 9. [Version 3] doi: 10.1101/2023.02.08.527750

Fig. 5. TAT-5/ATP9A, NDK-1/NME1, and APC-2/ANAPC2 are required for the integrity of the migrating male nematode gonad.

Fig. 5

(A) Males homozygotic for tat-5(tm1741), ndk-1(ok314), apc-2(ok1657), and lin-40(gk255) have gonads that were not connected to the exterior similar to that of lnkn-1(sy1596). For each genotype, the controls were siblings carrying the genetic balancer and were either the wild-type or heterozygous for the locus (n ≥19, ****: p<0.0001, chi-square test). (B–E) Detached linker cells (LC) were found in males carrying tat-5(tm1741), ndk-1(ok314), or apc-2(ok1657), similar to that of lnkn-1(sy1596). Images are of animals in the L4 larvae stage, with lag-2p::YFP (syIs128) (colored in green) labeling the detached linker cell (LC). The posterior end of the remaining gonad is outlined in a white dashed line. (B) lnkn-1(sy1596). (C) tat-5(tm1741). (D) ndk-1(ok314). (E) apc-2(ok1657).