Table 2 -.

Control CSF patient information

Control sample 1: 60-year-old male with 2 years of progressive spasticity (bilateral lower extremities, progressed to left upper extremity). Neurologic exam notable initially for spasticity and upper motor neuron signs, but no lower motor neuron signs. Initial EMG with no abnormal spontaneous activity and MRI brain, C-, T- spine unremarkable. CK mildly elevated at 304. CSF with 2 WBC (ref 0–5), prot 52 (ref 14–45), glc 61 (ref 50–80), OCB neg, <0.0 IgG synthesis rate. Mayo autoimmune encephalopathy evaluation of serum and CSF with no informative autoantibodies detected. Glycine receptor antibody also negative in serum (not tested in CSF). Wash University neuromuscular antibody panel on serum also unremarkable. Ultimately progressed, and EMG findings with upper and lower motor neuron findings; diagnosed with amyotrophic lateral sclerosis.

Control sample 2: 54-year-old male followed for postherpetic neuralgia. CAF with WBC 0, prot 38, glc 65, OCB 0, <0.0 IgG synthesis rate. CSF culture negative, HSV1/2 Ab screen IgG <0.34, HSV Type 1 Ab IgG 0.0, HSV 1 and or 2 Abs IgM 0.19, VZV IgG <10, VZV IgM 0.02, VZV PCR not detected, CMV IgM <8, HSV 6 Ab IgM by IFA <1:20. Note- all these results are within normal limits. No send out antibody testing done because no concern for immune-mediated process.

Control sample 3: 58-year-old female with diffuse paresthesia and pain, diagnosed with small fiber neuropathy, pernicious anemia and positive GAD65 antibody (without neurologic manifestations). CSF WBC 5, prot 37, glc 53, OCB negative (note: matched bands in serum and CSF), IgG synthesis rate <0.0, Mayo Paraneoplastic Autoantibody Evaluation from CSF positive for GAD65 Ab assay 0.04 nmol/L (ref 0.02), negative for AGNA1, Amphiphysin Ab, ANNA1, ANNA2, ANNA3, CRMP5 IgG, PCA1, PCA2, PCA-Tr. Mayo Autoimmune dysautonomia eval (serum) with AChR Ganglionic Neuronal Ab 0.03 nmol/L (ref <0.02), GAD65 Ab 20.1 nmol/L (ref <0.02), negative for ANNA1, Striational Ab, N-type Calcium Channel, Ach Receptor binding Ab, Neuronal VGKC Ab, P/Q Type Calcium channel. Intrinsic factor blocking antibody positive. GAD65 Ab at ARUP >250 IU/mL (ref 0–5)