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. 2023 Jan 31;11:e14785. doi: 10.7717/peerj.14785

Figure 2. Vitamin D receptor (VDR) knockdown attenuated the brown adipocyte differentiation of C3H10T1/2 cells.

Figure 2

C3H10T1/2 cells treated with small-interfering RNA (siRNA) were differentiated into mature brown adipocytes. (A) Western blot analysis of VDR and α/β-tubulin on day zero. (B) Representative images of Oil Red O staining in C3H10T1/2 cells after differentiation. Scale bars, 100 µm. (C) mRNA levels of Fabp4, Plin, and Ucp1 after differentiation of C3H10T1/2 cells (n = 3). (D) Western blot analysis of UCP1 and α/β-tubulin on day 7. Representative images with three samples of each siRNA are shown. Data are shown as mean ± standard error of the mean. *** p < 0.001.