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. 2023 Jan 31;11:e14785. doi: 10.7717/peerj.14785

Figure 3. Effects of 1,25(OH)2D3 on the differentiation and viability of C3H10T1/2 cells.

Figure 3

(A) Images of Oil Red O staining in differentiated C3H10T1/2 cells (scale bars, 500 µm) and (B) associated quantitative analysis of the extracted signal by spectrophotometry (n = 4). (C) Dose-dependent effects of 1,25(OH)2D3 on Plin mRNA expression (n = 3). (D) Effect of 1,25(OH)2D3 on C3H10T1/2 viability. Pre-differentiated (Pre) or differentiated (Dif) C3H10T1/2 cells were treated with 100 pM or 1000 pM 1,25(OH)2D3 for 24 h (n = 4). Data are shown as mean ± standard error of the mean. *** p < 0.001 versus control (0 pM).