Figure 4. Human aging lungs show no increase in transcriptional noise but consistent depletion and enrichment of specific endothelial and immune cell types.

The increase in transcriptional noise associated with aging (Noise, left) and the cell type enrichment (Enrichment, right) values are shown for 30 human lung cell identities as detected in the human lung cell atlas (HLCA) and Raredon datasets (Raredon et al., 2019; Travaglini et al., 2020). For each cell type, its age-related increase in noise (difference in 1 ‍ − membership between old and young individuals per cell type) and the old/young odds ratio (OR) are shown. Only cell types whose enrichment/depletion is statistically significant in at least one of the datasets are shown, and the ORs associated with a p-value >0.01 are shown as a triangle. The color-bar for the enrichment is shown in a logarithmic scale.

Figure 4—figure supplement 1. Composition of the two single-cell RNA sequencing datasets of the human aging lung used in this figure.

The uniform manifold approximation and projection plots with the age and cell type identity annotations are shown for each tissue compartment (endothelial, epithelial, stromal, and immune) and each dataset separately.