| Genetic reagent (D. melanogaster; male) |
w1118 |
BDSC |
|
|
| Genetic reagent (D. melanogaster; male) |
Oregon-R |
BDSC |
|
|
| Genetic reagent (D. melanogaster; male) |
y1w1 |
BDSC |
|
|
| Genetic reagent (D. melanogaster; male) |
tup::GFP |
BDSC |
RRID:BDSC_81278
|
y w; PBac{y[+mDint2] w[+mC]=tup GFP.FPTB}VK00031/TM6C, Sb |
| Genetic reagent (D. melanogaster; male) |
Amph::GFP |
Kyoto Stock Center |
CPTI-002789 |
|
| Genetic reagent (D. melanogaster; male) |
piwi::GFP |
Kyoto Stock Center |
CPTI-003588 |
|
| Genetic reagent (D. melanogaster; male) |
Akr1B::GFP |
Kyoto Stock Center |
CPTI-002728 |
|
| Genetic reagent (D. melanogaster; male) |
CG3902::GFP |
Kyoto Stock Center |
CPTI-100001 |
|
| Genetic reagent (D. melanogaster; male) |
comr z2-1340
|
White-Cooper |
FBal0144389 |
|
| Antibody |
Anti-Fasciclin III, mouse monoclonal |
Developmental Studies Hybridoma Bank |
DSHB:7G10; RRID:AB_528238
|
1:50 |
| Antibody |
Chick polyclonal anti-GFP |
Abcam |
ab13970 |
1:10,000 |
| Antibody |
Goat anti-chicken Alexafluor 488 |
Invitrogen |
A11039 |
1:200' |
| Antibody |
Goat anti-mouse Alexafluor 568 |
Invitrogen |
A11004 |
1:100' |
| Antibody |
Sheep Anti-FITC, horseradish peroxidase |
Roche |
11426338910 |
1:2000 |
| Antibody |
Sheep Anti-Digoxygenin, horseradish peroxidase |
Roche |
11207733910 |
1:1500 |
| Antibody |
Sheep Anti-digoxygenin, alkaline phosophatase |
Roche |
11093274910 |
1:2000 |
| Antibody |
mouse anti coracle monoclonal |
Developmental Studies Hybridoma Bank |
DSHB: C615.16 |
1:20 |
| Recombinant DNA reagent |
piwi (plasmid containing cDNA) |
BDGP DGC library |
GM05853 |
FBcl0142639 |
| Recombinant DNA reagent |
eya (plasmid containing cDNA) |
BDGP DGC library |
GH05272 |
FBcl0108545 |
| Recombinant DNA reagent |
f-cup (plasmid containing cDNA) |
BDGP DGC library |
GH09045 |
FBcl0128895 |
| Recombinant DNA reagent |
loqs (plasmid containing cDNA) |
BDGP DGC library |
RE14437 |
FBcl0204474 |
| Recombinant DNA reagent |
geko (plasmid containing cDNA) |
BDGP DGC library |
RE30284 |
FBcl0192532 |
| Recombinant DNA reagent |
Vsx1 (plasmid containing cDNA) |
BDGP DGC library |
SD01032 |
FBcl0286608 |
| Chemical compound, drug |
DAPI |
Millipore Sigma |
1023627600 |
1:1000 (of 2–5 mg/ml) |
| Chemical compound, drug |
Vectashield |
Vector Labs |
H-1000 |
|
| Chemical compound, drug |
GMM tissue clearing reagent |
Gary Struhl |
|
2 g Canada Balsam in 1 ml methyl salicylate |
| Chemical compound, drug |
Rhodamine tyramide |
Thermo Scientific |
46406 |
1:1000 |
| Chemical compound, drug |
Fluorescein tyramide |
Thermo Scientific |
46410 |
1:1500 |
| Commercial assay or kit |
Stellaris RNA FISH Probe Designer |
Biosearch Technologies Inc. |
|
|
| Commercial assay or kit |
10 X Genomics Chromium Controller; Next GEM Single Cell3’ v3.1 |
https://www.10xgenomics.com/product-catalog
|
1000269 |
|
| Software, algorithm |
Seurat 4.0.5 |
Satija et al., 2015; Hao et al., 2015
|
|
|
| Software, algorithm |
Monocle3 |
https://cole-trapnell-lab.github.io/monocle3/; Trapnell et al., 2014
|
|
|
| Software, algorithm |
Venny 2.1 |
https://bioinfogp.cnb.csic.es/tools.html
|
|
|
| Other |
Olympus Bx50 microscope |
Olympus |
|
|
| Other |
20 x/0.60 NA objective |
Olympus |
|
|
| Other |
10 x/0.30 NA objective |
Olympus |
|
|
| Other |
40 x/0.75 NA objective |
Olympus |
|
|
| Other |
KY F75U Camera |
JVC |
|
|
| Other |
Orca-05G Camera |
Hamamatsuu |
|
|
| Other |
63 x/1.4 NA objective |
Zeiss |
|
|
| Other |
LSM800 confocal |
Zeiss |
|
|
| Other |
63 x/1.4 NA objective |
Leica |
|
|
| Other |
Stellaris Confocal |
Leica |
|
|
