Figure 5. Midgut cell types with defining genes.

(A) Finer clustering of the midgut cluster results in three subclusters. (B) Gene expression dot plot of the two midgut subclusters that are presumably developing smooth muscles and digestive cells of the midgut. The size of the dots shows how much of a cluster expresses a gene; the colour shows how much of the expression of a gene is confined to a cluster (see Materials and methods). (C) Some of the genes shown to be differentially expressed in the two subclusters, plotted on the UMAP representation. (D) The location (upper panel) and an example cell (lower panel) of the subcluster located in the animal parapodia. Scale bars: upper panel - 25μm, lower panel - 5μm. (E) Cells belonging to the two cell types (left panels) and the genes differentiating them (centre and right panels) are visualised in the animal volume, with colour representing gene expression overlayed on the electron microscopy (EM) plane view. Scale bar: 10μm.