Table 4.

Sequential extraction of Zn, Cu and Mn from faecal samples collected on day 14.

Extraction steps	Elements	IL	IH	OL	OH	P values
		Mean ± SE (mg kg−1)				Form	Dose	Form × dose
Step 1: Exchangeable, water- and acid-soluble	Zn	115 ± 12.4	119 ± 20.0	113 ± 12.8	114 ± 7.9	0.767	0.852	0.900
	Cu	2.44 ± 0.093	2.67 ± 1.112	2.32 ± 0.172	2.43 ± 0.143	0.163	0.185	0.672
	Mn	307 ± 5.2	295 ± 8.0	310 ± 12.1	330 ± 16.0	0.102	0.732	0.165
Step 2: Reducible	Zn	138 ± 7.0	149 ± 23.2	147 ± 12.6	143 ± 4.0	0.902	0.745	0.539
	Cu	8.36 ± 0.500	7.91 ± 0.391	7.90 ± 0.456	7.76 ± 0.285	0.378	0.396	0.650
	Mn	64.8 ± 3.77	60.1 ± 2.96	62.1 ± 2.83	63.0 ± 2.27	0.988	0.529	0.355
Step 3: Oxidizable	Zn	15.8 ± 3.29	25.4 ± 2.32	20.0 ± 1.42	19.0 ± 1.01	0.012*	0.736	0.898
	Cu	22.1 ± 0.74	22.3 ± 0.85	21.1 ± 0.94	22.1 ± 0.89	0.565	0.551	0.701
	Mn	12.1 ± 2.16	9.99 ± 1.425	12.6 ± 0.88	10.1 ± 0.82	0.831	0.096	0.865
Step 4: Residual	Zn	5.81 ± 0.716	5.13 ± 0.374	4.57 ± 0.465	4.54 ± 0.261	0.054	0.428	0.463
	Cu	6.61 ± 0.143	6.90 ± 0.285	6.01 ± 0.893	6.70 ± 0.362	0.391	0.293	0.660
	Mn	2.46 ± 0.160	1.95 ± 0.149	1.98 ± 0.185	1.80 ± 0.130	0.042*	0.027*	0.263

Significant values are in bold.

OH organic minerals offered at higher doses, OL organic minerals offered at lower doses, IH inorganic minerals offered at higher doses, IL inorganic minerals offered at lower doses. The symbol ‘*’ indicate statistical significance of ANOVA test at p-value < 0.05.