Skip to main content
. 2023 Feb 16;14:886. doi: 10.1038/s41467-023-36483-3

Fig. 5. Inhibiting MAT2A decreases ammonium levels and restores differentiation capacity in progeric mouse myoblasts.

Fig. 5

a ATP levels in response to insulin and Akt2 inhibition. (*p = 0.02, **p = 0.00076, ***p = 0.0002, and ns represents p > 0.37) statistical significance using two-way ANOVA with Tukey’s multiple comparisons test. b ATP levels in response to MAT2A inhibitor FIDAS V. c ATP levels in LM cells with Mat2a knockdown. d Ammonium levels in response to insulin and Akt2 inhibition. (*p < 0.04 and **p < 0.001) statistical significance using two-way ANOVA with Tukey’s multiple comparisons test. e Ammonium levels in response to MAT2A inhibitor FIDAS V. For plots b and e statistical significance using two-way ANOVA with Sidak’s multiple comparisons test. (*p < 0.04, ****p < 0.0001, and ns represents p = 0.99). f Ammonium levels in LM cells with Mat2a knockdown. For c and f plots statistical significance using one-way ANOVA with Tukey’s multiple comparisons test (****p < 0.0001). g, h Level of s-adenosylmethionine (SAM) and s-adenosylhomocysteine (SAH) at the indicated conditions. Statistical significance using one-way ANOVA with Dunnett’s multiple comparisons test (****p < 0.0001). i Immunostaining for myosin heavy chain (MyHC) (red) and actinin (green) showing skeletal muscle differentiation (scale bar = 50 µm). WM: green bars, LM: red bars, LMN: blue bars, and LM + shMat2a-1,2: yellow bars. n = 5 animals per cohort. Data in bar graphs are presented as mean ± SD.