Fig. 6. NANOG restores markers of methionine catabolism and glycolysis in the TA muscle of LAKI mice.

Elvax (100 μl) loaded with Dox (25 mg) was implanted around the right TA muscle of LAKI mice to induce NANOG expression, while the left limb served as an internal control (Elvax, no Dox). Two weeks later, the Elvax was removed and the TA muscle was evaluated 3 weeks later. a Western blot and band quantification for MAT2A; GAPDH served as loading control b Ammonium levels. c Western blot and band quantification for SLC42A2; GAPDH served as loading control. d, e Effect of NANOG on s-adenosyl methionine (SAM) and s-adenosylhomocysteine (SAH) levels. f Western blot and band quantification for InsR; GAPDH served as loading control. g PK activity levels. WT: green boxes, L: red boxes, and LN: blue boxes. n = 5 animals per cohort. For the boxplots, the top and bottom lines of each box represent the 75th and 25th percentiles of the samples, respectively. The line inside each box represents the median of the samples. The upper and lower lines above and below the boxes are the whiskers. Statistical significance using one-way ANOVA with Tukey’s multiple comparisons test.