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. 2023 Feb 16;13:2811. doi: 10.1038/s41598-023-28773-z

Figure 3.

Figure 3

A crude extract of EL001672 and 1′-O-methyl-averantin suppress expression of cancer stemness markers. CSC221 cells were treated for 48 h with a crude extract of EL001672 or 1′-O-methyl-averantin for mRNA and protein expressions. (A,B) Quantitative analysis of mRNA encoding cancer stem markers aldehyde dehydrigebase-1 (ALDH1), cluster of differentiation 133 (CD133), CD44, Lgr5, Musashi-1, and EphB1. CSC221 cells were treated for 48 h with a crude extract of EL001672 or 1′-O-methyl-averantin. (C,E) Immunoblots are shown. Relative expression of each target protein after cells were exposed to a crude extract of EL001672 (D) or 1′-O-methyl-averantin (F). 10, 5 and 2.5 µg/ml 1′-O-methyl averantin represent a concentration of 25.90, 12.95 and 6.47 µM, respectively. Data represent the mean ± SEM. *p < 0.05; **p < 0.01 (compared with DMSO-treated CSC221 cells).