Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Feb 16;13:2811. doi: 10.1038/s41598-023-28773-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Effect of a crude extract of EL001672 and 1′-O-methyl-averantin on the NOTCH signaling pathway. (A,B) Quantitative analysis of Notch-1 and Hes-1 (hairy and enhancer of split-1) mRNA expression by CSC221 cells treated with the indicated concentrations of crude extract or 1′-O-methyl-averantin and incubated 48 h. (C,D) CSL (CBF1/Su(H)/Lag-1)-luc and Hes-1 reporter assays in HEK293T cells treated with 2.5, 5, or 10 μg/mL crude extract, 1′-O-methyl-averantin, 5 μg/mL GANT61 (a Gli inhibitor), and Vismodegib-GDC-0449 (a SMO inhibitor) and incubated 48 h. 10, 5 and 2.5 µg/ml 1′-O-methyl averantin represent a concentration of 25.90, 12.95 and 6.47 µM, respectively. Data represent the mean ± standard error of the mean. *p < 0.05; **p < 0.01; ***p < 0.001; difference compared with each groups.