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. 2023 Feb 3;10:980433. doi: 10.3389/fmolb.2023.980433

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Differential expression analysis of small RNAs. (A) Differential expression analysis of miRNAs and (B) piRNAs. Volcano plots depict significant differences in the pre-operation urinary or plasma EVs as compared to the post-operation EVs, and in small RNA libraries prepared from tumor (T_S) and normal prostate tissues (N_S). Venn diagrams show the numbers of small RNAs overexpressed in tumor tissues vs. normal prostate tissues (Up in T_S vs. N_S), decreased in the post-operation urinary EVs as compared to pre-operation urinary EVs and decreased in the post-operation plasma EVs as compared to pre-operation plasma EVs (Log2FC >1 and adj.p-value ≤0.05).