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Validation of selected biomarker candidates in urinary EVs from an independent cohort of 20 PC patients by RT-ddPCR. Paired dot plots show the copy number of RNA biomarkers per ml of urine collected before and after radical prostatectomy in 20 PC patients. Wilcoxon matched-paired signed rank test was used to assess the statistical significance of the differences between groups. p-value < 0.05 was considered significant.
