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. 2023 Feb 16;14:872. doi: 10.1038/s41467-023-36522-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Two photon intravital imaging analysis of infiltrated neutrophils (Ly6G-AF488) in renal blood vessels (Texas Red) of live mice (glomeruli and tubulointerstitial) after saline or pristane treatment. Scale bar, 30 μm. b Quantitative analysis of infiltrated neutrophils in glomeruli and tubulointerstitial of mice after saline or pristane treatment. n = 6 mice. c Immunofluorescence staining of Ly6G in glomeruli from Ms4a3-tdTomato (Ms4a3-Td) mice after pristane treatment. Scale bar, 30 μm. d Quantitative analysis of Ly6G⁺ and Ms4a3-tdTomato⁺ cell numbers in each glomerulus and percentage of Ms4a3-tdTomato⁺ Cells. Representative of three independent experiments, and each point represents one glomerulus with the mean being represented by a horizontal line. e, f Intravital imaging of the kidneys from pristane-treated Ms4a3-Td mice revealing the release of DNA (Sytox Green, green) from tdTomato⁺ cells (Red). Time-lapse images are shown of DNA that was released in the form of punctate particles (e) or mesh-like structures (f). Scale bar, 20 μm. 5 μm (enlarged). Representative of two independent experiments. g Quantitative analysis of PE-Ly6G⁺SG⁺ cells/10 min in kidney of PIL and Gsdmd^−/− mice by intravital imaging. n = 6 mice. Representative of three independent experiments. Data are presented as mean ± SD. Significance was examined with unpaired two-sided Student’s t test (b, d, g).