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. 2023 Feb 16;14:890. doi: 10.1038/s41467-023-36441-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — CryoEM map A and model B for the C5b8-CD59 nanodisc complex. Complement proteins are colored (C5b, grey; C6, blue; C7, orange; C8α, pink; C8β, magenta; C8γ light purple). CD59 is cyan. The membrane, not visible in the sharpened map, is schematized for reference. To improve the resolution for CD59, the density for C5b, C6 and C8γ was subtracted from the consensus map (A) and refined. C Model for CD59 and interaction interface with C8α overlayed on the density subtracted focus refined map.