Figure 1.

Liver biopsy (all originally magnified principal images). Hematoxylin and eosin staining showed the discorded architecture of liver lobules and the formation of pseudo-lobules (A × 40), balloon-like hepatic cells, giant cell changes of hepatocytes and bile plugs formation in hepatocytes and capillary bile ducts (B × 200), and ductular proliferation, moderate portal-tract inflammation, and fibrosis in the portal area (C × 100). PAS staining identified small vacuoles in partial hepatocytes (D × 200). Formation of bridging-like fibrosis and pseudo-lobule was highlighted by Masson staining (E × 50). The preserved reticular scaffold structure was displayed by reticulin staining (F × 40). Obvious hyperplasia of bile ducts along the margins of pseudo-lobule was showed by immunohistochemical staining of CK7 (G × 100). DCDC2 immunohistochemical staining showed weaker expression in patient 1 (H × 200) than in normal control (I × 200).