Table 1.

Anopheles species used in this study with length and G+C variation of cloned ITS sequences

Species	Geographic origin	No. of individuals	No. of clones	ITS1 length (bp)	ITS1 G+C%	ITS1 mean p-distance	ITS2 length (bp)	ITS2 G+C%
An. farauti	Rabaul (PNG)	12	22	841–1138	54–58	0.0076	554*	69
	Northern Territory (AUS)	1	3	1204–1491	58–59.8	0.0017	549	68.9
	Central Province (PNG)	1	4	1167–1194	57.2–57.3	0.0034	562*	68.5
An. irenicus	Guadalcanal (Sol Is)	3	9	1197–1584	57.6–60	0.0045	560*	69.3
An. farauti 6	Irian Jaya (Indo)	2	3	1169–1175	55.9–56	0.0046	549	66.5
An. hinesorum	Queensland (Aus)	4	10	1162–1486	55.6–57.8	0.0068	555	66.3
An. torresiensis	Queensland (Aus)	5	8	1183–1200	62.4–63.2	0.0058	533	71.3
An. koliensis	Madang Province (PNG)	2	3	1467–1469	61.2	0.0143	591	67.9
	Central Province (PNG)	1	2	1465–1480	61.1	0.0055	–	–
An. punctulatus	Madang Province (PNG)	3	7	1590–1799	63–64.1	0.0059	551	67.3
An. sp. nr punctulatus	Southern Highlands (PNG)	3	7	1706–2006	60.5–61.3	0.0038	557	64.8
An. clowi	Irian Jaya (Indo)	1	3	548–731	54.4–55.1	0.0030	579*	64.9
An. annulipes sp. l	Karumba (Aus)	1	3	1370	53.1–53.2	0.0024	415*	65.3

Note: Aus Australia, PNG Papua New Guinea, Sol Is Solomon Islands, Indo Indonesia

* ITS2 sequences isolated in this study; all others from Beebe et al. (1999)