FIGURE 3.

Effect of palbociclib and fulvestrant on bsAb-mediated T cell expansion. eFluor™ 670⁺ T cells and (A,B) PC3-PSCA/PSMA Luc+ or (C,D) LNCaP-PSCA Luc+ cells were incubated with or without CD3-PSCA bsAb. Palbociclib and/or fulvestrant were added at indicated concentrations. After 96 h, numbers of eFluor™ 670⁺ T cells were determined by flow cytometry using the MACSQuant^® Analyzer. (A,C) Each diagram shows average T cell counts ± SEM of triplicates for one T cell donor. (B,D) Graphs summarize relative T cell counts ± SEM of three different T cell donors. T cell numbers in the presence of tumor cells and bsAb (“bsAb”) were equalized to 100%. (*p ≤ 0.05, ***p ≤ 0.001 compared to control sample “bsAb”; One-way ANOVA with posthoc Dunnett multiple comparison test).