FIGURE 6.

Effect of palbociclib and fulvestrant on IFN-γ release of TM-engaged UniCAR T cells. UniCAR T cells and (A,B) PC3-PSCA/PSMA Luc+ or (C,D) LNCaP-PSCA Luc+ cells were incubated with or without 30 nM of PSCA TM in the presence or absence of palbociclib and/or fulvestrant. After 96 h, IFN-γ concentrations in co-culture supernatants were analyzed by ELISA. (A,C) Each diagram shows average IFN-γ concentration ± SEM of triplicates for one T cell donor, respectively. (B,D) Graphs summarize relative IFN-γ release ± SEM of three different T cell donors. IFN-γ concentrations in the presence of UniCAR T cells, tumor cells and TM (“TM”) were equalized to 100%. (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 compared to control sample “TM”; One-way ANOVA with posthoc Dunnett multiple comparison test).