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. 2023 Feb 17;9:67. doi: 10.1038/s41420-023-01366-5

Fig. 2. IL-35 producing B cells are the dominant regulatory population during colitis.

Fig. 2

A–C Cells were isolated from mesenteric lymph nodes (MLNs) (A), Peyer’s patches (Patches) (B), and colorectal lamina propria (LP) (C) from 2.5% dextran sulfate sodium (DSS)-treated wildtype (WT) mice at day 0, 4, 7, and 10 post-disease onset. The percentage and absolute number of IL-35 producing B cells (CD19+ IL-12A+ EBI3+ cells) were determined by flow cytometry (n = 3 per group). D Expression levels of IL-35 producing B cells (CD20+ IL-12A+ EBI3+ cells) in formalin-fixed paraffin-embedded Peyer’s patches tissue sections from DSS-treated mice were determined by immunofluorescence staining (n = 6 per group). A–D Two-tailed unpaired Student’s t-test. Data are shown as mean ± standard error of the mean (SEM) and are representative of at least three independent experiments (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001).