Table 2.
Primer Sequence: Primer sequence of mice genotyping and qRT-PCR.
| Primer | Sequence(5′-3′) | Usage |
|---|---|---|
| muMT Mutant R | TTGTGCCCAGTCATAGCCGAAT | Mice genotyping |
| muMT common | CCGTCTAGCTTGAGCTATTAGG | Mice genotyping |
| muMT WT R | GAAGAGGACGATGAAGGTGG | Mice genotyping |
| IL12A Mutant F | CTGAATGAACTGCAGGACGA | Mice genotyping |
| IL12A Mutant R | ATA CTT TCT CGG CAG GAG CA | Mice genotyping |
| IL12A Wild Type F | CAG CAT GTG TCA ATC ACG | Mice genotyping |
| IL12A Wild Type R | TCA CCA TGT CAT CTG TGG | Mice genotyping |
| EBi3 Mutant R | GCC AGA GGC CAC TTG TGT AG | Mice genotyping |
| EBi3 Common | AAC CTC AGG CCA GGC AGT | Mice genotyping |
| EBi3 Wild Type R | TTC CGT AGG CCA TGT AGG AC | Mice genotyping |
| IL12p35 F | GACCTGTTTACCACTGGAACTA | qRT-PCR |
| IL12p35 R | GATCTGCTGATGGTTGTGATTC | qRT-PCR |
| EBi3 F | CTTCTCTCTCAAGTACCGACTC | qRT-PCR |
| EBi3 R | TTATGGGGTGCACTTTCTACTT | qRT-PCR |
| β-actin F | CCAGCCTTCCTTCTTGGGTATG | qRT-PCR |
| β-actin R | TGTGTTGGCATAGAGGTCTTTACG | qRT-PCR |