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. 2023 Feb 16;14:874. doi: 10.1038/s41467-023-36600-2

Fig. 3. DdCBE_Ss can install disease-associated mtDNA mutations in previously inaccessible GC sites.

Fig. 3

a Use DdCBE_Ss to install disease-associated target mutations in human mtDNA (R, arginine; H, histidine; A, alanine; V, valine). b, c Mitochondrial base editing efficiencies of HEK293T cells treated with ND4-DdCBE (b), ND6-DdCBE (c), the background color of disease-associated target sites is orange, the gray nucleotide are part of the TALE binding site, Ddd_Ss1: Ddd_Ss (T26I + T77I + T110I); Ddd_Ss2: Ddd_Ss (T26I); Ddd_Ss3: Ddd_Ss (T77I); Ddd_Ss4: Ddd_Ss (T110I); Ddd_Ss5: Ddd_Ss (T77I + T110I); Dead-Ddd_Ss5: Ddd_Ss (E44A + T77I + T110I). Shown are mean ± SD; n = 3 independent experiments. The transfection time was 3 days. DdCBE_Ss5 was compared against DdCBE_Ss. P values were calculated by Student’s unpaired two-tailed t-test. d, e Oxygen consumption rate (OCR) (d) and relative values of respiratory parameters (e) in HEK293T cells treated with the ND6-DdCBE_Ss5 or dead ND6-DdCBE_Ss5. Shown are mean ± SD; n = 3 independent experiments. P values were calculated by Student’s unpaired two-tailed t-test. Source data are provided as a Source Data file.