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. 2023 Feb 17;14:898. doi: 10.1038/s41467-023-36607-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Experimental setup. b Sucrose gradient profiles of in vitro reconstitution samples incubated for 0 min (black) or 3 min (red), respectively. c In vitro translation assay indicating the relative activity of subunits after the indicated time of incubation under step 1 conditions plus subsequent incubation under step 2 conditions for 90 min. Data are presented as mean values based on n = 2 technical replicates. d In the center, a color-coded 2D map of the 23S rRNA with the individual domains labeled from I–VI (5S, 5S rRNA). Cryo-EM maps of selected states derived from the step 1 reaction after 3 min of incubation appear in the same color-code. e Nomenclature for states occurring during early and late 50S assembly. States in bold are shown in (d). d, 23S rRNA domain; C, 50S core; CP, central protuberance; H68, helix 68. Source data are provided as a Source Data file.