Skip to main content
. 2023 Feb 3;10:1029021. doi: 10.3389/fmed.2023.1029021

FIGURE 3.

FIGURE 3

mTOR inhibition in RA synovial fibroblasts decreases cellular migration. Bar graphs showing the measured secretion of (A) MCP-1, RANTES, and IL-8 from RASF (n = 6–13) and mRNA expression of (B) VCAM-1 and ICAM-1 in RASF (n = 7–14) treated with TNFα (1 ng/ml) and Rapamycin (100 nM) alone or in combination for 24 h. Gene expression analysis quantified using Real-time PCR. Fold increase compared to endogenous controls (RPLPO and HPRT1). Fold change MFI expression of ICAM-1 on RASF (n = 7). (C) Representative photomicrographs and (D) accompanying bar graph demonstrating cells repopulating the wound to represent migration. Images were taken of RASF (n = 8) after being treated with TNFα (1 ng/ml) and Rapamycin (100 nM) alone or in combination for 24 h. Values expressed as mean ± SEM, Wilcoxon signed rank test used for RASF. *p < 0.05, **p < 0.01, ***p < 0.001.