Figure 5.

hPCLS demonstrate retained morphological features over 4 weeks in culture. Photomicrographs (×10) taken at day 1 post acclimation (4 days following slice creation for fresh, and 2 days post thaw for Cryo 1 and Cryo 2) show normal morphology, regardless of group. At the final harvest time point (day 29) of the 4-week culture period all 3 groups again show a highly preserved lung architecture and cellular viability as seen in healthy lung tissue. Note the cells morphologically compatible with macrophages (arrows) in the intra-alveolar spaces at day 29. N = 3/group per time point.