Figure 8.

Retained viability and immune responsiveness in cryopreserved hPCLS from additional donor lungs. The F&C hPCLS were cultured at ALI using DMEM:F-12 medium for up to 14 days. During the culture period, a subset of hPCLS were treated with 5 µg/ml LPS or VC (culture medium) at days 6 and 13. After 24 h of treatment, the lysate and medium samples were collected for determination of protein content (A), tissue viability (B, C), IL-6 (D), TNF-α (E), and MMP-3 (F). The data are presented as AVE ± SEM, N = 3/group per time point.