Figure 3.

Direct synthesis of labeled NADP from the ³²P-NAD⁺ reaction product. (a) Proposed reaction for the synthesis of labeled NADP from ³²P-NAD⁺. Position of the ³²P label is indicated by a star. (b) ADP, ATP, NAD⁺, and NADP standards were resolved with TLC using PEI cellulose F plates and imaged following excitation at 254 nm. (c) Samples (1 μL) from the NADK enzymatic reaction were obtained at indicated time points, resolved with TLC, and radioactivity was detected with phosphorimaging. Leading edges were measured from respective origins (dashed circles).