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Schematic workflow for the generation of the sex-specific protein coronas on three different types of NPs. NPs with and without protein corona were physicochemically characterized by dynamic light scattering (DLS), ζ-potential analysis, and transmission electron microscopy (TEM). The formed protein coronas were profiled by liquid chromatography coupled to mass spectrometry (LC-MS/MS) to investigate sex-specific differences in protein coronas.
