Fig. 3.

Tumor exosomal cSERPINE2 targeted macrophages to promote proliferation and metastasis of breast cancer cells. a THP-1 cells were treated with PMA, followed by the treatment of CM or exosomes derived from tumor cells as indicated treatments to obtain TAMs. Then tumor cells cocultured with these TAMs were used to subsequent experiments. b, c Phenotype analysis of exosomes derived from MCF-7 and EO771 cells using electron microscopy and NanoSight nanoparticle tracking analysis. Scale bar, 100 nm. d CD63 and CD81 expression in exosomes derived from MCF-7 and EO771 cells were detected by Western blotting. e The relative expression of cSERPINE2 in exosomes derived from MCF-7 and EO771 cells as indicated treatments. f The relative expression of cSERPINE2 in THP-1 or BMDM derived TAMs as indicated treatments. g-j The proliferation and invasion of MCF-7 and EO771 cells as indicated treatments in vitro were evaluated using EdU and transwell invasion assays, respectively. Scale bar, 20 μm. Data are presented as the means ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001