Fig. 2.

Effects of hMSC conditioned medium treatment on AHR to methacholine challenge in 21 day old mice with or without prior CPAP (7 days) treatment (A). AHR was assessed using the precision-cut lung slice method by determining the changes in airway lumen size in response to increasing doses of methacholine. The smaller lumen size at increasing methacholine concentrations signifies increased AW reactivity. Note, AHR following CPAP (open circles) was reversed by treatment with conditioned medium from three different hMSC donors (Donor 2200, 2216, and 2222). Representative images for each treatment group at baseline (0 μM) and the highest (8 μM) dose of methacholine are also provided (B). *indicates the slope of the response was significantly different from the slope of control (ctrl, no CPAP) mice (*p < 0.05).