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. 2023 Jan 26;43(1):22–42. doi: 10.1080/10985549.2022.2160605

FIG 8.

FIG 8

CircIFNGR2 acted as a sponge for miR-378 in OVCA to regulate ST5 expression. (A) Predicted binding sites of miR-378 to wild-type or mutant circIFNGR2 and ST5 sequences. (B and C) Analysis of luciferase activity in OVCA cells co-transfected with miR-378 mimic, miR-NC and wild-type or mutant luciferase reporter vectors. (D) RNA pull-down assays were performed in A2780 and OV90 cells to confirm the direct binding of miR-378 to circIFNGR2 or ST5. (E) Relative expression levels of miR-378 in A2780 and OV90 cells were assessed by qRT-PCR. (F) A2780 and OV90 cells were transfected with oe-NC, oe-circIFNGR2, oe-circIFNGR2+mimic and oe-circIFNGR2+nc mimic, and the expression levels of miR-378 were examined by qRT-PCR. (G) Differential expression of miR-378 and ST5 in ovarian cancer patients and healthy controls was evaluated by qRT-PCR. (H) qRT-PCR was arranged to detect the differential expression of miR-378 and ST5 in OVCA cell lines (A 2780 and OV90) and normal ovarian epithelial cell lines (IOSE80). (I) Correlation between miR-378 and circIFNGR2 or ST5 was analyzed in OVCA tissues by Pearson’s analysis. **P < 0.01, ***P < 0.001 and ***P < 0.01.