Table 1. . Polymerase chain reaction primer sets for detection of SARS-CoV-2 variants of concern.
Primer name | Position | Forward sequence 5′–3′ | Position | Reverse sequence 5′–3′ | Amplicon Size (bp) |
---|---|---|---|---|---|
G1_KFSHRC | 21645–21664 | ACACTAATTCTTTCACACGT | 22101–22082 | TCAAGGTCCATAAGAAAAGG | 457 |
G2(A)_KFSHRC | 22733–22754 | TGCTTTACTAATGTCTATGCAG | 23226–23207 | GACTCAGTAAGAACACCTGT | 494 |
G2(B)_KFSHRC | 22882–22903 | TCTTGATTCTAAGGTTGGTGGT | 23519–23500 | CCCCTATTAAACAGCCTGCA | 638 |
G2(C)_KFSHRC | 23296–23315 | TCCACAGACACTTGAGATTC | 23873–23854 | CTATTCCAGTTAAAGCACGG | 578 |
G3_KFSHRC | 24368–24387 | GACTCACTTTCTTCCACAGC | 24993–24974 | TCAGGTTGCAAAGGATCATA | 626 |