Skip to main content
. 2023 Feb 6;33(3):533–542.e5. doi: 10.1016/j.cub.2022.12.051
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
MAC265 Rat monoclonal: Infection thread matrix glycoprotein CarboSource http://glycomics.ccrc.uga.edu/wall2/antibodies/antibodyHome.html
LM5 Rat monoclonal: Pectic polysaccharide ((1-4)-β-D-galactan) PlantProbes https://plantcellwalls.leeds.ac.uk/science/antibodies/
LM6 Rat monoclonal: Pectic polysaccharide ((1-5)-α-L-arabinosyl) PlantProbes https://plantcellwalls.leeds.ac.uk/science/antibodies/
LM19 Rat monoclonal: Un-esterified homogalacturonan PlantProbes https://plantcellwalls.leeds.ac.uk/science/antibodies/ RRID:AB_2734788
LM20 Rat monoclonal: Methyl-esterified homogalacturonan PlantProbes https://plantcellwalls.leeds.ac.uk/science/antibodies/ RRID:AB_2734789
2F4 Mouse monoclonal: 'egg box'' dimer conformation of homogalacturonan PlantProbes http://glycomics.ccrc.uga.edu/wall2/antibodies/antibodyHome.html
LM2 Rat monoclonal: Arabinogalactan-proteins (AGPs) PlantProbes https://plantcellwalls.leeds.ac.uk/science/antibodies/
LM14 Rat monoclonal: Arabinogalactan-proteins (AGPs) PlantProbes https://plantcellwalls.leeds.ac.uk/science/antibodies/
LM30 Rat monoclonal: Arabinogalactan-proteins (AGPs) PlantProbes https://plantcellwalls.leeds.ac.uk/science/antibodies/
LM25 Rat monoclonal: Xyloglucan PlantProbes https://plantcellwalls.leeds.ac.uk/science/antibodies/
AlexaFluor 546 goat anti-rat Thermo Fisher RRID:AB_2534125
AlexaFluor 546 goat anti-mouse Thermo Fisher RRID:AB_2534071
Protein A-gold 5 nm PAG5; CMC, Utrecht, The Netherlands N/A
Goat-anti Rat IgG 12 nm gold Jackson Immuno Research RRID:AB_2338272
Bacterial and virus strains
Sinorhizobium meliloti 2011 Lab stocks N/A
Agrobacterium rhizogenes strain ARqua1 Lab stocks N/A
Chemicals, peptides, and recombinant proteins
Calcofluor White Fluka Cat#18909-100ML-F
X-Gluc (5-bromo-4-chloro-3-indoxyl-b-D-GlcA, cyclohexylammonium salt Carl Roth Cat#Art.Nr.0018.3
Ruthenium red Sigma-Aldrich Cat#R2751
Toluidine blue Sigma-Aldrich Cat#89640
glutaraldehyde 25% Carl Roth Art.Nr.4157.3
Paraformaldehyde (PFA) Sigma-Aldrich Cat#P-6148
xyloglucanase (XYLOGLUCANASE (GH5) (Paenibacillus sp.) Megazyme Cat#no. E-XEGP
PECTATE LYASE (Apergillus sp.) Megazyme Cat#no. E-PCL YAN
Critical commercial assays
Bsa I enzyme for Goldengate reactions New England BIOLABS Cat#R3733
Bpi I enzyme for Goldengate reactions New England BIOLABS Cat#R35395
Technovit 7100 MORPHISTO Cat#12227.K0500
Technovit 8100 MORPHISTO Cat#12226.K0500
Experimental models: Organisms/strains
Medicago truncatula cultivar Jemalong Heritage Seeds Pty, Adelaide, AU Jemalong
Medicago truncatula ecotype R108 Tnt1 insertion line (sypme1) Medicago truncatula Mutant Database: https://medicago-mutant.dasnr.okstate.edu/mutant/index.php NF2281
Medicago truncatula ecotype R108 Tnt1 insertion line (npl) Rival et al.18 NF18556
Oligonucleotides
Primers for genotyping, SYMPEM-F: CTCTACACAACTGA
ATCTACAATCTTTTCC		 This paper N/A
Primers for genotyping, SYMPEM-R: CAGAATATTCTATCATA
ACCCAATGCAACC		 This paper N/A
Primers for genotyping, TNT-R: TGTAGCACCGAGATA
CGGTAATTAACAAGA		 This paper N/A
Primers for in situ, SyPME1-situ-F: TGTGTTTGGTC
ACTCTCGCAC		 This paper N/A
Primers for in situ, SyPME1-situ-R: ACAATTGAACCTTCT
AATCCTTCTATACAT		 This paper N/A
Sequence for NPL-RNAi design: TCGGTAGACGTGCAATT
GGAGGTAAAGATGGAAAA
TATTACATGGTCATTGACT
CAAGTGATGACCCTGTGA
ATCCTAAGCCAGGAACATT
AAGACATGCTGTTATCCAACAA
GAGCCTTTATGGATCATTTTCAA
GCATGACATGGTGATCAAACT
AAAGATGGATCTTCTCATGAAT
TCTTTCAAAACAATTGATGGTA
GAGGTGTAAATGTACACATTGCT
GAAGGACCATGTATTAGAATACA
AGAAAAGACCAACATCATAATT
CATGGTATACACATTCA
TCATTGTGTACGAG		 This paper N/A
Recombinant DNA
Promoter activity:ProNPL::GUS//ProUBI::NLS-2xmCherry This paper N/A
Promoter activity:
ProSyPME1::GUS//ProUBI::NLS-2xmCherry		 This paper N/A
Localization analysis:
ProNPL::NPL-GFP//ProUBI::NLS-2xmCherry		 This paper N/A
Localization analysis:
ProNPL::SyPME1-GFP//ProUBI::NLS-2xmCherry		 This paper N/A
Localization analysis, PME activity measurement:
ProUBI::SyPME1-GFP//ProUBI::NLS-2xmCherry		 This paper N/A
Phenotypical analysis, PME activity measurement:
ProNPL::AtPMEI12-GFP//ProUBI::NLS-2xmCherry		 This paper N/A
Phenotypical analysis:
ProUBI::NPL-RNAi//ProUBI::NLS-2xmCherry		 This paper N/A
Phenotypical analysis:
ProLeEXT1::NPL-RNAi//ProUBI::NLS-2xmCherry		 This paper N/A
Phenotypical analysis:
ProAtPEP::NPL-RNAi//ProUBI::NLS-2xmCherry		 This paper N/A
Promoter activity:
ProLeEXT1::GUS//ProUBI::NLS-2xmCherry		 This paper and Goodchild and Bergersen 24 N/A
Promoter activity:
ProAtPEP::GUS//ProUBI::NLS-2xmCherry		 This paper and Szczyglowski et al.25 and Peaucelle et al.26 N/A
Empty vector: Dummy//ProUBI::NLS-2xmCherry This paper N/A
Software and algorithms
All box plots have been plotted using Graphpad Prism Graphpad Prism 8 https://www.graphstats.net/
All statistical tests have been carried out using Graphpad Prism Graphpad Prism 8 https://www.graphstats.net/
Adobe Photoshop (Figure editing and typesetting) Photoshop CS6 https://www.adobe.com/products/photoshop.html
ZEN (blue version) ZEN 3.0 https://www.zeiss.com/microscopy/int/products/microscope-software/zen.html
ImageJ Schindelin et al. 38 https://imagej.nih.gov/ij/
IMARIS Imaris 7.2.3 https://imaris.oxinst.com/
Phylogenetic tree was built by iTOL iTOL v6 https://itol.embl.de/