Appendix 1—key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| strain, strain background (Drosophila melanogaster) | Canton S | Martin Heisenberg | N.A. | |
| strain, strain background (Drosophila melanogaster) |
20xUAS-CsChrimson- mVenus attP18 |
Klapoetke et al., 2014; PMID: 24509633 |
N.A. | |
| strain, strain background (Drosophila melanogaster) |
10XUAS-Chrimson88- tdTomato attP1 |
Klapoetke et al., 2014; PMID: 24509633 |
N.A. | |
| strain, strain background (Drosophila melanogaster) | 13XLexAop2-IVS- ChrimsonR-mVenus- p10 attP18 |
Vivek Jayaraman | N.A. | |
| strain, strain background (Drosophila melanogaster) | 20XUAS-syn21-mScarlet- opt-p10 su(Hw)attp8 |
Glenn Turner | N.A. | |
| strain, strain background (Drosophila melanogaster) |
pJFRC200-10xUAS- IVS-myr::smGFP-HA in attP18 |
Nern et al., 2015; PMID: 25964354 | N.A. | |
| strain, strain background (Drosophila melanogaster) |
pJFRC225-5xUAS- IVS-myr::smGFP-FLAG in VK00005 |
Nern et al., 2015; PMID: 25964354 | N.A. | |
| strain, strain background (Drosophila melanogaster) | pBPhsFlp2::PEST in attP3 | Nern et al., 2015; PMID: 25964354 | N.A. | |
| strain, strain background (Drosophila melanogaster) | pJFRC201-10XUAS-FRT>STOP > FRT-myr::smGFP-HA in VK0005 | Nern et al., 2015; PMID: 25964354 | N.A. | |
| strain, strain background (Drosophila melanogaster) | pJFRC240-10XUAS-FRT>STOP > FRT-myr::smGFP-V5-THS-10XUAS-FRT>STOP > FRT-myr::smGFP-FLAG_in_su(Hw)attP1 | Nern et al., 2015; PMID: 25964354 | N.A. | |
| strain, strain background (Drosophila melanogaster) | LexAop2-DA2m VK00005 | Sun et al., 2020; PMID: 33087905 | N.A. | |
| strain, strain background (Drosophila melanogaster) | MB043-split-LexA | This paper | N.A. | Available from Aso lab |
| strain, strain background (Drosophila melanogaster) |
empty-split-GAL4 (p65ADZp attP40, ZpGAL4DBD attP2) |
Seeds et al., 2014; PMID: 25139955 |
N.A. | |
| strain, strain background (Drosophila melanogaster) | MB032B split-GAL4 | Aso et al., 2014; PMID: 25535793 | N.A. | |
| strain, strain background (Drosophila melanogaster) | MB043C split-GAL4 | Aso et al., 2014; PMID: 25535793 | N.A. | |
| strain, strain background (Drosophila melanogaster) | MB109B split-GAL4 | Aso et al., 2014; PMID: 25535793 | N.A. | |
| strain, strain background (Drosophila melanogaster) | MB213B split-GAL4 | Aso et al., 2014; PMID: 25535793 | N.A. | |
| strain, strain background (Drosophila melanogaster) | MB315C split-GAL4 | Aso et al., 2014; PMID: 25535793 | N.A. | |
| strain, strain background (Drosophila melanogaster) | SS33917 split-GAL4 | This paper | N.A. | Available from Aso lab |
| strain, strain background (Drosophila melanogaster) | SS45234 split-GAL4 | This paper | N.A. | Available from Aso lab |
| strain, strain background (Drosophila melanogaster) | SS67221 split-GAL4 | This paper | N.A. | Available from Aso lab |
| strain, strain background (Drosophila melanogaster) | UAS-TeNT |
Keller et al., 2002: PMID: 11810637 |
N.A. | |
| antibody | anti-GFP (rabbit polyclonal) | Invitrogen | A11122 RRID:AB_221569 |
1:1000 |
| antibody | anti-Brp (mouse monoclonal) |
Developmental Studies Hybridoma Bank | nc82 RRID:AB_2341866 |
1:30 |
| antibody | anti-ChAT (mouse monoclonal) |
Developmental Studies Hybridoma Bank | ChAT4B1 RRID:AB_528122 |
1:50 |
| antibody | anti-HA-Tag (mouse monoclonal) |
Cell Signaling Technology | C29F4; #3724 RRID:AB_10693385 |
1:300 |
| antibody | anti-FLAG (rat monoclonal) |
Novus Biologicals | NBP1-06712 RRID:AB_1625981 |
1:200 |
| antibody | anti-V5-TAG Dylight-549 (mouse monoclonal) |
Bio-Rad | MCA2894D549GA RRID:AB_10845946 |
1:500 |
| antibody | anti-mous IgG(H&L) AlexaFluor- 568 (goat polyclonal) |
Invitrogen | A11031 RRID:AB_144696 |
1:400 |
| antibody | anti-rabbit IgG(H&L) AlexaFluor-488 (goat polyclonal) |
Invitrogen | A11034 RRID:AB_2576217 |
1:800 |
| antibody | anti-mouse IgG(H&L) AlexaFluor-488 conjugated (donkey polyclonal) |
Jackson Immuno Research Labs |
715-545-151 RRID:AB_2341099 |
1:400 |
| antibody | anti-rabbit IgG(H&L) AlexaFluor-594 (donkey polyclonal) |
Jackson Immuno Research Labs |
711-585-152 RRID:AB_2340621 |
1:500 |
| antibody | anti-rat IgG(H&L) AlexaFluor-647 (donkey polyclonal) |
Jackson Immuno Research Labs |
712-605-153 RRID:AB_2340694 |
1:300 |
| antibody | anti-Mouse IgG (H&L) ATTO 647 N (goat polyclonal) |
ROCKLAND | 610-156-121 RRID:AB_10894200 |
1:100 |
| antibody | anti-rabbit IgG (H+L) Alexa Fluor 568 (goat polyclonal) |
Invitrogen | A-11036 RRID:AB_10563566 |
1:1000 |
| chemical compound, drug | 3-Octanol | Sigma-Aldrich | 218405 | |
| chemical compound, drug | 4-Methylcyclohexanol | VWR | AAA16734-AD | |
| chemical compound, drug | Pentyl acetate | Sigma-Aldrich | 109584 | |
| chemical compound, drug | Ethyl lactate | Sigma-Aldrich | W244015 | |
| chemical compound, drug | Paraffin oil | Sigma-Aldrich | 18512 | |
| software, algorithm | ImageJ and Fiji | NIH Schindelin et al., 2012 |
https://imagej.nih.gov/ij/ http://fiji.sc/ | |
| software, algorithm | MATLAB | MathWorks | https://www.mathworks.com/ | |
| software, algorithm | Adobe Illustrator CC | Adobe Systems | https://www.adobe.com/products/illustrator.html | |
| software, algorithm | GraphPad Prism 9 | GraphPad Software | https://www.graphpad.com/scientific-software/prism/ | |
| software, algorithm | Python | Python Software Foundation |
https://www.python.org/ | |
| software, algorithm | neuPrint | HHMI Janelia | https://doi.org/10.25378/janelia.12818645.v1 | |
| software, algorithm | Cytoscape | Shannon et al., 2003 | https://cytoscape.org/ | |
| software, algorithm | NeuTu | Zhao et al., 2018 |
https://github.com/janelia-flyem/NeuTu; janelia-flyem, 2021 |
|
| software, algorithm | ScanImage | Vidrio Technologies | https://vidriotechnologies.com/ | |
| software, algorithm | VVDveiwer | HHMI Janelia | https://github.com/takashi310/VVD_Viewer | |
| other | Grade 3 MM Chr Blotting Paper |
Whatmann | 3030–335 | Used in glass vials with paraffin-oil diluted odours |
| other | mass flow controller | Alicat | MCW-200SCCM-D | Mass flow controller used for the olfactory arena |