Figure	Genotype
Figure 1C–G, Figure 1—figure supplement 1	Canton S
Figure 1H	w/w, 20xUAS-CsChrimson-mVenus attP18;+/Gr64f-GAL4;+/Gr64f-GAL4
Figure 2A–C	w/w, 20xUAS-CsChrimson-mVenus attP18;;+/MB043C-split-GAL4 w/w, 20xUAS-CsChrimson-mVenus attP18;+/MB213B-split-GAL4 w/w, 20xUAS-CsChrimson-mVenus attP18;;+/MB312C-split-GAL4 w/w, 20xUAS-CsChrimson-mVenus attP18;MB109B/MB315C-split-GAL4 w/w, 20xUAS-CsChrimson-mVenus attP18;+/ Empty-split-GAL4
Figure 2D	w/+;Empty-split-GAL4/UAS-TNT (II) w/+;MB196B/UAS-TNT (II) w/+;MB043C/UAS-TNT (II)
Figure 3 Figure 3—figure supplement 3	w/w,13XLexAop2-IVS-ChrimsonR-mVenus-p10 attP18, 20XUAS-syn21 mScarlet-opt-p10 su(Hw)attp8; SS01308-split-GAL4/MB043-split-LexA
Figure 3—figure supplement 1	w/w,13XLexAop2-IVS-ChrimsonR-mVenus-p10 attP18, 20XUAS-syn21 mScarlet-opt-p10 su(Hw)attp8; +/MB043-split-LexA w/w,13XLexAop2-IVS-ChrimsonR-mVenus-p10 attP18, 20XUAS-syn21 mScarlet-opt-p10 su(Hw)attp8; MB319C-split-GAL4/MB043-split-LexA w/w,13XLexAop2-IVS-ChrimsonR-mVenus-p10 attP18, 20XUAS-syn21 mScarlet-opt-p10 su(Hw)attp8; SS01308-split-GAL4/MB043-split-LexA w/w,13XLexAop2-IVS-ChrimsonR-mVenus-p10 attP18, 20XUAS-syn21 mScarlet-opt-p10 su(Hw)attp8; SS67221-split-GAL4/MB043-split-LexA
Figure 3—figure supplement 2	w/w,13XLexAop2-IVS-ChrimsonR-mVenus-p10 attP18, 20XUAS-syn21 mScarlet-opt-p10 su(Hw)attp8; MB319C-split-GAL4/MB043-split-LexA
Figure 4E	w/w, pJFRC200-10xUAS-IVS-myr::smGFP-HA in attP18; pJFRC225-5xUAS-IVS-myr::smGFP-FLAG in VK00005/SS67221-split-GAL4
Figure 4F	pBPhsFlp2::PEST in attP3;; pJFRC201-10XUAS-FRT>STOP > FRT-myr::smGFP-HA in VK0005, pJFRC240-10XUAS- FRT >STOP > FRT-myr::smGFP-V5-THS-10XUAS-FRT>STOP > FRT-myr::smGFP-FLAG in su(Hw)attP1/SS67221-split-GAL4
Figure 5	13XLexAop2 IVS p10 ChrimsonR mVenus trafficked in attP18/+; 58E02-LexAp65 in attP40 /VT026646-p65ADZp in attP40 (ss45234-split); pJFRC28-10XUAS-IVS-GFP-p10 in su(Hw)attP1 /VT029309-ZpGdbd in attP2 (ss45234-split)
Figure 6, Figure 6—figure supplement 1	w/w, 10XUAS-Chrimson88-tdTomato attP18; 13F02-LexAp65 attP40; LexAop2-DA2m VK00005/MB043C-split-GAL4 w/w, 10XUAS-Chrimson88-tdTomato attP18; 13F02-LexAp65 attP40; LexAop2-DA2m VK00005/MB213B-split-GAL4 w/w, 10XUAS-Chrimson88-tdTomato attP18; 13F02-LexAp65 attP40; LexAop2-DA2m VK00005/MB032B-split-GAL4 w/w, 10XUAS-Chrimson88-tdTomato attP18; 13F02-LexAp65 attP40; LexAop2-DA2m VK00005/MB109B-split-GAL4 w/w, 10XUAS-Chrimson88-tdTomato attP18; 13F02-LexAp65 attP40; LexAop2-DA2m VK00005/MB315C-split-GAL4 w/w, 10XUAS-Chrimson88-tdTomato attP18; 13F02-LexAp65 attP40; LexAop2-DA2m VK00005/MB312C-split-GAL4 w/w, 10XUAS-Chrimson88-tdTomato attP18; 13F02-LexAp65 attP40; LexAop2-DA2m VK00005/SS33917-split-GAL4 w/w, 10XUAS-Chrimson88-tdTomato attP18; 13F02-LexAp65 attP40; LexAop2-DA2m VK00005/SS67221-split-GAL4
Figure 7	w/+;SS67221/+ w/+; SS67221/UAS-TNT (II) w/+;SS45234/+w/+; SS45234/UAS-TNT (II) w/+;Empty-split-GAL4/TNT (II)SS67221/TNT
Figure 7—figure supplement 1A	w/w, 20xUAS-CsChrimson-mVenus attP18;+/ Empty-split-GAL4 w/w, 20xUAS-CsChrimson-mVenus attP18;+/SS67221-split-GAL4
Figure 7—figure supplement 1B	w/+;SS67221/+ w/+; SS67221/UAS-TNT (II) w/+;Empty-split-GAL4/TNT (II)SS67221/TNT
Figure 8, Figure 8—figure supplement 1	w/w, 20xUAS-CsChrimson-mVenus attP18;+/+;+/MB043C-split-GAL4 w/w, 20xUAS-CsChrimson-mVenus attP18;+/SS33917-split-GAL4 w/w, 20xUAS-CsChrimson-mVenus attP18;+/SS67221-split-GAL4 w/w, 20xUAS-CsChrimson-mVenus attP18;+/MB032B-split-GAL4 w/w, 20xUAS-CsChrimson-mVenus attP18;+/MB109B-split-GAL4 w/w, 20xUAS-CsChrimson-mVenus attP18;+/+;+/MB315C-split-GAL4 w/w, 20xUAS-CsChrimson-mVenus attP18;+/MB312C-split-GAL4 w/w, 20xUAS-CsChrimson-mVenus attP18;+/MB213B-split-GAL4