Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Mus musculus) | B6.SJL-Slc6a3tm1.1(Cre)Bkmm/J (DATIRES-Cre) | Bäckman et al., 2006 | RRID:IMSR_JAX:006660 | |
| Genetic reagent (M. musculus) |
Rims1tm3Sud/J (RIM1αβfl/fl) |
Kaeser et al., 2008 | RRID:IMSR_JAX:015832 | |
| Genetic reagent (M. musculus) |
Rims2tm1.1Sud/J (RIM2αβγfl/fl) |
Kaeser et al., 2011 | RRID:IMSR_JAX:015833 | |
| Genetic reagent (M. musculus) |
C57BL/6Ntac-Syt1tm1a(EUCOMM)Wtsi/WtsiCnrm (Syt-1fl/fl) |
Zhou et al., 2015 | RRID:IMSR_EM:06829 | The identifier refers to the line before flp recombination |
| Cell line (Homo sapiens) | HEK293T cells | ATCC | Cat#: CRL-3216; RRID:CVCL_0063 | |
| Recombinant DNA reagent | pAAV2/5-syn-DIO-RIM-PRM-PDZ-PxxP-C2A-BirA | This study | LK18005; lab plasmid code (LPC) p864 | This reagent was used to generate AAV viruses and can be obtained from the corresponding author |
| Recombinant DNA reagent | pAAV2/5-syn-DIO- ELKS2β-BirA | This study | LK17008; LPC p857 | This reagent was used to generate AAV viruses and can be obtained from the corresponding author |
| Recombinant DNA reagent | AAV2/5-syn-DIO-CaVβ4-BirA | This study | LK19004; LPC p868 | This reagent was used to generate AAV viruses and can be obtained from the corresponding author |
| Recombinant DNA reagent | AAV2/5-syn-DIO-BirA-tdTomato | This study | LK17011; LPC p860 | This reagent was used to generate AAV viruses and can be obtained from the corresponding author |
| Antibody | Anti-HA (rabbit polyclonal) | Cell Signaling Technology | CAT# 5017; RRID:AB_10693385, lab antibody code (LAC) A40 |
Immunofluorescence (IF) (1:500) |
| Antibody | Anti-HA (mouse monoclonal) | BioLegend | CAT# 901501; RRID:AB_2565006, LAC A12 |
IF (1:500) Western blot (WB) 1:500 |
| Antibody | Anti-tyrosine hydroxylase (guinea pig polyclonal) | SySy | CAT# 213 104; RRID:AB_2619897, LAC A111 |
IF (1:1000) |
| Antibody | Anti-Bassoon (mouse monoclonal) | Millipore | CAT# ADI-VAM-PS003-F; RRID:AB_11181058, LAC A85 | IF (1:500) |
| Antibody | Anti-Neuroplastin (goat polyclonal) | R&D Systems | CAT# AF5360; RRID:AB_2155920, LAC A253 |
IF (1:500) |
| Antibody | Anti-red fluorescent protein (rabbit polyclonal) | Rockland | CAT#600-401-379; RRID:AB_2209751, LAC A81 | WB (1:1000) |
| Antibody | Anti-β-actin (mouse monoclonal) | Sigma-Aldrich | CAT# A1978; RRID:AB_476692, LAC A127 |
WB (1:10,000) |
| Antibody | Anti-pyruvate carboxylase (rabbit polyclonal) | Novus | CAT# NBP1-49536G; RRID:AB_11016707, LAC A252 | This antibody was used for depletion as described in the 'Materials and methods' |
| Software, algorithm | Prism9 | GraphPad | RRID:SCR002798; https://www.graphpad.com/scientific-software/prism |
|
| Software, algorithm | Cytoscape v 3.8.2 | Cytoscape | RRID:SCR003032; http://cytoscape.org/ |
|
| Software, algorithm | SEQUEST PRO | Thermo Fisher | https://scicrunch.org/resolver/SCR_014594 | |
| Software, algorithm | MATLAB code for object analysis | Liu, 2021 | https://github.com/kaeserlab/3DSIM_Analysis_CL |