Figure 2. Snf1 prevents transient TORC1 restimulation by glutamine in glucose- and glutamine-starved cells.

(A–F) Exponentially growing snf1^as cells were starved for 20 min for nitrogen and glucose (-N, -C) and then restimulated for the times indicated, in the absence (DMSO) or presence of 2NM-PP1, with 2% glucose (Glc) (A, B), 2% glucose and 3.3 mM glutamine (Gln) (C, D), or 3.3 mM glutamine (E, F). Immunoblot analyses were performed as in Figure 1A and the relative TORC1 activities in (A), (C), and (E), were quantified as in Figure 1B and are shown in (B), (D), and (F), respectively (n=3; + SEM; unpaired Student’s t-test, *p≤0.05, ***p≤0.0005). The online version of this article includes the following source data for Figure 2—source data 1, quantification of blots for graphs shown in (B, D and F); Figure 2—source data 2, uncropped blots shown in (A, C and E); Figure 2—source data 3, raw blots shown in (A, C and E).