Figure 1.
Effects of Nef expression on GFAP protein expression in human astrocytes. Human astrocytic cell lines SVGA (A) or U373 (C) were plated in a 6-well plate at a density of 2.5 x 105 cells/well for SVGA or 3.0 x 105 cells/well for U373, transfected with 2.5 μg pcDNA3 plasmid (C3) or pNef.Myc plasmid (Nef). The cell lysates were analyzed by Western blotting (A & C) against an anti-GFAP, Nef, GAPDH, or β-actin antibody. GFAP expression was quantitated, normalized to GAPDH or β-actin, and expressed as the relative values to the control (rel) (B & D). untx: untransfected cells. The data were Mean ± SEM and representative of three independent experiments (n=3).