Figure 10.

Effects of Nef on astrocyte proliferation. U373 were transfected with pC3 Nef (A, B, & E), or infected with VSVG/GAGi and GAGi.Nef- (C, D & F) and cultured for 48 hr. Brightfield and FITC images were taken at 20x magnification and cells in the brightfield were counted using Image J software (A -D). The MTT assay was performed, and absorbance was measured at 595 nm (background subtraction at 655nm (E &F). The data were Mean ± SEM and representative of three independent experiments (n=3).