Figure 2.

Effects of Nef expression on GFAP expression in human astrocytes in the context of HIV infection. U373 were infected with VSVG/GAGi and GAGi.Nef- (A-C) or NLGi or NLGi.Nef- (D-F). The cells were cultured for 48 hr, the GFP+ cells were estimated under a fluorescence microscope and expressed as a ratio to the total number of cells (A & D); or the cells were harvested for cell lysates and Western blotting against an anti-GFAP, Nef, GAPDH, or β-actin antibody (B & E). GFAP expression was quantitated, normalized to GAPDH or β-actin, and expressed as the relative values to the control (rel.) (C & F). The data were Mean ± SEM and representative of three independent experiments (n=3).