Figure 4.

Effects of Nef expression on GFAP mRNA expression in astrocytes. U373 (A & B), mouse primary astrocytes (C), human primary fetal astrocytes (D) were transfected with C3 or Nef (A) or infected with VSVG/GAGi and GAGi.Nef- (B-D). Total RNA was extracted and GFAP mRNA were determined by qRT-PCR. Threshold cycle values were normalized to the mRNA level of GFP which was included as the transfection efficiency control (A) or expressed from VSVG/GAGi and GAGi.Nef- and then normalized to the qRT-PCR control GAPDH and expressed as the relative values to the control C3 (A) or VSVG/GAGi (B-D) for infection efficiency and GAPDH mRNA levels. The data were Mean ± SEM and representative of three independent experiments (n=3).