Figure 5.

Effects of Nef expression on the GFAP Promoter activity. U373 were transfected with pGFAP.luc plasmid, cultured for 16 hr, split into two equal samples, and infected with VSVG/GAGi and GAGi.Nef-. The cells were cultured for 48 hr and harvested for Western blotting against an anti-Nef, or β-actin antibody (A), or harvested for the luciferase reporter gene assay (B). The relative luciferase activity was normalized to the protein concentration. The data were Mean ± SEM and representative of three independent experiments (n=3).