Table 2.

Fibulin-5-siRNA screening.

Group	Internal reference	Ct	ΔCt	ΔΔCt	2-ΔΔCt
A	11.94	15.75	3.81	0.00	1.00
B	12.13	16.05	3.91	0.10	0.95
C	13.98	17.72	3.73	-0.08	1.09
D	12.44	19.09	6.64	2.83	0.15
E	11.76	16.35	4.58	0.78	0.59
F	12.53	16.55	4.03	0.22	0.87

A: blank group; B: Lipofectamine 2000 only; C: negative siRNA control group; D: Fibulin-5-siRNA-1; E: Fibulin-5-siRNA-2; F: Fibulin-5-siRNA-3. ΔCt = target gene Ct − internal reference Ct. ΔΔCt is the target gene ΔCt in the experimental sample minus target gene ΔCt in the reference sample; assuming the amplification efficiency of PCR is close to 100%, the relative sample template amount = 2 − ΔΔCt.