Table 4.
The primers for PCR amplification.
| NO. | Target | Sequence | TM |
|---|---|---|---|
| 1 | hsa-miR-25-5p_R-1 | F: AACAAGAGGCGGAGACTTGG R: GTCGTATCCAGTGCAGGGT |
60 |
| 2 | hsa-miR-873-5p_L+1R-3 | F: AACAAGTGCAGGAACTTGTGAG R: GTCGTATCCAGTGCAGGGT |
60 |
| 3 | hsa-miR-340-5p | F: AACACGCTTATAAAGCAATGAGACT R: GTCGTATCCAGTGCAGGGT |
60 |
| 4 | hsa-miR-142-3p_L-2R-1 | F: AACACGCTAGTGTTTCCTACTTTAT R: GTCGTATCCAGTGCAGGGT |
60 |
| 5 | hsa-miR-181c-5p_R-1 | F: AACAAGAACATTCAACCTGTCGG R:GTCGTATCCAGTGCAGGGT |
60 |
| 6 | hsa-miR-324-3p_L-3R+1 | F:AACAAGACTGCCCCAGGTG R:GTCGTATCCAGTGCAGGGT |
60 |
Note: F, Forward Primer; R, Reverse Primer; TM, Annealing Temperature.