Fig. 5.

Silencing of β-catenin alters mRNA and protein expression of circadian clock regulators. SW480 cells grown on the 12 well cell culture plates were transfected with β-catenin-specific siRNA at the indicated concentration or with nonspecific, scrambled siRNA (Scr); Veh, vehicle. mRNA (left panels) and protein (right panels) expression of β-catenin (A), Bmal1 (B), Clock (C), Per1 (D), Per2 (E), Cry1 (F), and (Cry2) (G) were assayed and quantified. Blots illustrate representative data. Band intensity was assessed by densitometric analysis using Image J program. p indicates the level of statistical significance after β-catenin silencing as compared to the Scr group. Values (means ± SD) are expressed as fold change compared with Scr; n = 3–6