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. 2023 Feb 17;13:2858. doi: 10.1038/s41598-023-29958-2

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Expression analysis of TET1 to 3 using RT-qPCR. Expression levels of TET2 (0.26-fold, p < 0.01) and TET3 (0.23-fold, p = 0.03) were significantly lower in CRC tissues, when compared to that in NC tissues (a), whereas no significant difference was observed in TET1 expression (p = 0.89). In the NC tissue (b), no significant differences were detected in the expression levels of TET1 (p = 0.72), TET 2 (p = 0.21), and TET 3 (p = 0.37). In CRC tissue (c), there was a lower expression of TET2 in Ob-CRC, compared to that in the nOb-CRC group (0.27-fold, p = 0.04). *p < 0.05, **p < 0.01, NS no significant. RT-qPCR real-time quantitative polymerase chain reaction, NC adjacent noncancerous, CRC colorectal cancer, TET ten-eleven translocation, ACTB β-actin, Ob-CRC obese patients with colorectal cancer, nOb-CRC non-obese patients with colorectal cancer.