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. 2022 Dec 14;17(3):326–339. doi: 10.1038/s41396-022-01348-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Change of the magnetic response (Cmag) of three randomLy selected MAG insertion mutants with sequential culture passages. Arrows indicate the timepoints at which the genomes were re-sequenced. b TEM micrographs of the cells at timepoint 1 (MAG) and timepoint 17, after the loss of magnetosomes (nonMAG). Scale bars: 0.5 µm. c Read coverage normalized to the library size of the MAG and nonMAG mutants. The gene maps show the insertion positions of the MAG cassette (highlighted by red rectangle) within the genome. d Bar chart showing the percentage of reads indicating the MAG cassette excision and the cassette presence.