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. 2022 Dec 20;17(3):354–370. doi: 10.1038/s41396-022-01345-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Sampling was conducted in the campos rupestres grasslands ecoregion (left). Vellozia epidendroides (center) specimens were collected in patches of shallow soil. Barbacenia macrantha (right) was found in a rocky area, where it grows over exposed rocks. B Community composition inferred from 16S rRNA gene ASVs at the phylum level. Samples were grouped according to their environment. Bar heights are proportional to the relative abundance of the phylum. Low abundance phyla (relative abundance < 2%) were grouped under the “Other” category. C Maximum-likelihood phylogenetic tree of the bacterial MAGs presented in this study, rooted at the Patescibacteria clade. The innermost ring indicates the phylum associated with each node. The center ring shows the genomic GC content. The outermost ring represents the scaled means of log-transformed relative genomic coverages across the four environments. D Weighted average community identity (WACI) computed from 16S rRNA gene ASV data. The blue and green dashed lines represent the median intra-rank 16S rRNA gene identity at the genus and family levels, respectively. E Phylogenetic gain (PG) contributed by the MAGs to different taxa at the phylum, class, and order levels. Only taxa with PG higher than the following cut-offs are shown: 5% at the phylum level, 30% at the class level, and 40% at the order level. RX root (external), RN root (internal), SX stem (external), SN stem (internal), LX leaf (external), LN leaf (internal).