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. 2023 Feb 18;14:927. doi: 10.1038/s41467-023-36526-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Surfaces of PhuN-C (top) and PhuN-O (bottom) colored by electrostatic potential with their corresponding models on the left. b Electrostatic potential coloration of the interior and exterior phage nucleus surfaces created by PhuN assemblies. Surface charge calculation was done at pH 6.5 and includes the C-terminal tail tips. Red corresponds to negative and blue corresponds to positive charge. c Direct comparison of PhuN-C and PhuN-O. The asymmetric subunits in the tetramer are overlaid showing minor differences (light and dark gray) while the interaction with the neighboring β-hairpins differs dramatically, shifting by ~20 Å from near the C-terminus (PhuN-O) towards a flexible loop (PhuN-C).